mouse anti-chicken cd3 (clone ct-3)  (SouthernBiotech)

Journal: Veterinary Sciences

Article Title: Role of Spirulina platensis and Humic Acid in Mitigating Acute Cyclic Heat Stress: Effects on the Growth Performance, Meat Quality, Immunological Responses, and Tissue Histomorphology in Broiler Chickens

doi: 10.3390/vetsci12121187

Figure Lengend Snippet: Photomicrographs from chicken’s spleen immunostained with monoclonal antibody against CD3+ T cells surface antigen, showing the % of the expressed antigen as brown cytoplasmic staining reaction of moderate intensity (red arrows). Black arrows point to negative cells. ( A ) NEG CON, ( B ) POS CON, ( C ) SP, ( D ) HA, ( E ) SP+HA. Scale bars 20 μm.

Article Snippet: Two drops of the supersensitive mouse anti-Chicken CD3, clone CT-3 (Bio-Rad Lab., Dubai, UAE), and CD20 (ThermoFisher Scientific, Waltham, MA, USA) were applied to the sections.

Techniques: Staining

Journal: Frontiers in Immunology

Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

doi: 10.3389/fimmu.2025.1517697

Figure Lengend Snippet: List of antibodies.

Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

Techniques: Control

Journal: Frontiers in Immunology

Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

doi: 10.3389/fimmu.2025.1517697

Figure Lengend Snippet: Analysis of monocyte population enrichment following plastic adherence of PBMCs. (A) Gating strategy for flow cytometry analysis of CD3+ and MRC1LB+ cells. The mononuclear cell population was gated based on forward scatter (FSC)-A and side scatter (SSC)-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye (B) Representative flow cytometry dot plots of CD3 expression by live PBMCs and adherent PBMCs (n = 3). Numbers indicate the percentage of positive cells.

Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

Techniques: Flow Cytometry, Expressing

Journal: Frontiers in Immunology

Article Title: Generation and characterization of chicken monocyte-derived dendritic cells

doi: 10.3389/fimmu.2025.1517697

Figure Lengend Snippet: LPS-stimulated chicken MoDCs induced the proliferation of autologous T cells. Splenocytes from naïve chickens were isolated and CD4 T cells were enriched by magnetic selection. The CD4 T cells were then CFSE-labelled and co-cultured with allogeneic unstimulated or 6 h LPS-stimulated MoDCs. (A) Flow cytometry gating strategy. CFSE-stained cells were selected. The mononuclear cell population was gated based on FSC-A and SCC-A parameters and singlets were selected from the FSC-A versus FSC-H. Dead cells were excluded using a viability dye and CD3+ cells were gated based on CD3 versus FSC-H. (B) Representative histograms of unstimulated and LPS-stimulated CFSE-labeled T cells proliferation. Numbers on histograms indicate the percentage of proliferating T cells. (C) Proportions of proliferating T cells. A two-tailed paired t-test was used to determine statistical differences (*p<0.01). The data from two independent experiments are displayed (n=8).

Article Snippet: Mouse anti-chicken CD3 (Clone CT-3) , IgG1κ , PACBLU (Pacific BlueTM) , 8200-26 , Southern Biotech , 20 μg/mL.

Techniques: Isolation, Selection, Cell Culture, Flow Cytometry, Staining, Labeling, Two Tailed Test

Journal: Veterinary research

Article Title: Revealing novel and conservative CD8 + T-cell epitopes with MHC B2 restriction on ALV-J.

doi: 10.1186/s13567-024-01426-3

Figure Lengend Snippet: Figure 1 Detection of ALV-J shedding, ALV-J viremia, ALV-J antibody and T lymphocyte percentage of B2 haplotype chickens after ALV-J infection. Six chickens were randomly selected for sampling every 7 days post-infection (dpi). A ALV-J shedding was monitored via detecting the p27 expression levels in cloacal swabs. S/P value below 0.2 indicated negative ALV-J shedding. B ALV-J viremia was monitored via virus isolation. An S/P value above 0.2 indicated positive ALV-J viremia. C The ALV-J antibody level in the serum was monitored using the commercial ALV-J antibody test kit. An S/P value above 0.6 was considered ALV-J antibody positive. Five days before infection (dbi) and each week after infection, PBMCs derived from five chickens of infected and control groups were isolated to detect the T lymphocyte percentage, including the percentage of the CD4+CD3+T cell (D), the CD8α+CD3+T cell (E), and the CD3+CD4+CD8α+T cell (F). Each sample collected 1 × 105 cells for flow cytometric analysis. The one-way test was used for statistical comparison among A, B and C. And the unpaired Student t test was used for statistical comparison among D, E and F. * P < 0.05, ** P < 0.01.

Article Snippet: PBMCs (1 × 106) were incubated with mouse anti-chicken CD3 mAb (SouthernBiotech, Birmingham, USA), mouse anti-chicken CD4 mAb (SouthernBiotech, Birmingham, USA) and mouse anti-chicken CD8α mAb (SouthernBiotech, Birmingham, USA), respectively, at 4 °C for 30 min in the dark.

Techniques: Infection, Sampling, Expressing, Virus, Isolation, Derivative Assay, Control, Comparison

Journal: Veterinary research

Article Title: Revealing novel and conservative CD8 + T-cell epitopes with MHC B2 restriction on ALV-J.

doi: 10.1186/s13567-024-01426-3

Figure Lengend Snippet: Figure 1 Detection of ALV-J shedding, ALV-J viremia, ALV-J antibody and T lymphocyte percentage of B2 haplotype chickens after ALV-J infection. Six chickens were randomly selected for sampling every 7 days post-infection (dpi). A ALV-J shedding was monitored via detecting the p27 expression levels in cloacal swabs. S/P value below 0.2 indicated negative ALV-J shedding. B ALV-J viremia was monitored via virus isolation. An S/P value above 0.2 indicated positive ALV-J viremia. C The ALV-J antibody level in the serum was monitored using the commercial ALV-J antibody test kit. An S/P value above 0.6 was considered ALV-J antibody positive. Five days before infection (dbi) and each week after infection, PBMCs derived from five chickens of infected and control groups were isolated to detect the T lymphocyte percentage, including the percentage of the CD4+CD3+T cell (D), the CD8α+CD3+T cell (E), and the CD3+CD4+CD8α+T cell (F). Each sample collected 1 × 105 cells for flow cytometric analysis. The one-way test was used for statistical comparison among A, B and C. And the unpaired Student t test was used for statistical comparison among D, E and F. * P < 0.05, ** P < 0.01.

Article Snippet: APC-conjugated mouse anti-chicken CD3+, PE-conjugated mouse anti-chicken CD8α+, FITC-conjugated mouse anti-chicken CD4+, (SouthernBiotech, Birmingham, USA) were used in this study.

Techniques: Infection, Sampling, Expressing, Virus, Isolation, Derivative Assay, Control, Comparison